Mouse heavy chain variable regions: nucleotide sequence of a germ-line VHgene segment

Mouse heavy chain variable regions: nucleotide sequence of a germ-line VH gene segment.

We have constructed a library of Balb/c mouse embryo DNA in the vector Charon 4A. The library was searched for sequences homologous to the VH region of a cloned cDNA of the UPC10 heavy chain mRNA. In this paper, we describe the structure and the partial nucleotide sequence of one of such clones (VH441). The nucleotide sequence of this germ-line gene indicates that it encodes amino-acids 1-98 of...

Characterization of a complete immunoglobulin heavy-chain variable region germ-line gene of rainbow trout.

A germ-line heavy-chain variable region (VH) gene (RTVH431) has been isolated from a rainbow trout (Salmo gairdneri) and characterized by complete nucleotide sequencing. It is characteristic of VH, as shown by the conserved octamer and TATA motif in the 5' region, the heptamernonamer recombination signal sequence in the 3' region, and the 18-amino-acid-long hydrophobic leader interrupted by an ...

Nucleotide sequence of an anti-fluorescyl hapten antibody heavy chain variable region gene from a BALB/c mouse hybridoma cell line.

Nucleotide sequence of a new BALB/c mouse kappa light chain variable region gene.

We report here a new BALB/c mouse kappa light chain variable regk>n(Vk) genomic DNA sequence. Genbank search showed that this Vk gene has the highest homology (63%) with a human Vk gene EV15 (1). This Vk gene was cloned from the genomic DNA library of a murine hybridoma cell line designated 33.28 (2) by using a 2.7 kb Hindm fragment of mouse germline Jk region as probe (3). In hybridoma cell li...

The Complete Nucleotide Sequence of the Human Immunoglobulin Heavy Chain Variable Region Locus

The complete nucleotide sequence of the 957-kb DNA of the human immunoglobulin heavy chain variable (VH) region locus was determined and 43 novel VH segments were identified. The region contains 123 VH segments classifiable into seven different families, of which 79 are pseudogenes. Of the 44 VH segments with an open reading frame, 39 are expressed as heavy chain proteins and 1 as mRNA, while t...